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binary vector pbi121  (TaKaRa)


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    Structured Review

    TaKaRa binary vector pbi121
    Binary Vector Pbi121, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbi121+binary+vector/10__1016_slash_j__pmpp__2024__102341-66-10-13
    Average 86 stars, based on 1 article reviews
    binary vector pbi121 - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Cloning:

    Article Title: Changes in the promoter of a defender against apoptotic cell death gene affect its expression in upland cotton
    Article Snippet: The DAD1 (defender against apoptotic cell death) gene is a negative regulator of programmed cell death.. It plays important roles in plant growth, development, environmental adaptation, and aging.. We examined whether and how the expression of DAD1 gene is affected by the promoter changes in the allotetraploid upland cotton (Gossypium hirsutum L.).

    Clone Assay:

    Article Title: Interaction between two rice mitogen activated protein kinases and its possible role in plant defense
    Article Snippet: OsMPK20-4 and OsMPK3 were in-frame cloned in pECFP-N1 and pEYFP-N1 vectors (Clontech) to produce C-terminal CFP and YFP fluorescent fusion proteins (primer sequences mentioned in Additional file ). .. The cassette was also cloned into pBI121 binary vector (Clontech). ..

    Article Title: Methylation mediated by an anthocyanin, O -methyltransferase, is involved in purple flower coloration in Paeonia
    Article Snippet: .. PsAOMT and PtAOMT were cloned into the pBI121 binary vector (Clontech). ..

    Article Title: Cell cycle stage-specific differential expression of topoisomerase I in tobacco BY-2 cells and its ectopic overexpression and knockdown unravels its crucial role in plant morphogenesis and development.
    Article Snippet: DNA topoisomerases catalyze the inter-conversion of different topological forms of DNA.. Cell cycle coupled differential accumulation of topoisomerase I (Topo I) revealed biphasic expression maximum at S-phase and M/G1-phase of cultured synchronized tobacco BY-2 cells.. This suggested its active role in resolving topological constrains during DNA replication (S-phase) and chromosome decondensation (M/G1 phase).

    Plasmid Preparation:

    Article Title: Interaction between two rice mitogen activated protein kinases and its possible role in plant defense
    Article Snippet: OsMPK20-4 and OsMPK3 were in-frame cloned in pECFP-N1 and pEYFP-N1 vectors (Clontech) to produce C-terminal CFP and YFP fluorescent fusion proteins (primer sequences mentioned in Additional file ). .. The cassette was also cloned into pBI121 binary vector (Clontech). ..

    Article Title: Methylation mediated by an anthocyanin, O -methyltransferase, is involved in purple flower coloration in Paeonia
    Article Snippet: .. PsAOMT and PtAOMT were cloned into the pBI121 binary vector (Clontech). ..

    Article Title: Functional characterization of a type 2 metallothionein gene, SsMT2 , from alkaline-tolerant Suaeda salsa
    Article Snippet: .. The PCR fragments were digested with Bam HI and Sac I and then ligated into the Bam HI/ Sac I site of pBI121 binary vector (Takara, Tokyo, Japan), the plasmid DNAs of pBI121-SsMT2 was transformed into the Agrobacterium tumefaciens strain EHA105 (Takara, Tokyo, Japan) and then Arabidopsis (ecotype: Columbia) was transformed using the floral dip method . .. To examine the expression pattern of the SsMT2 gene in different organs of S . salsa plant, total RNA was isolated from roots, leaves, shoots, flowers and seeds respectively using RNeasy Plant Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions.

    Article Title: Mitochondria Change Dynamics and Morphology during Grapevine Leaf Senescence
    Article Snippet: .. For the expression of the GFP targeted to mitochondria, the β-GFP coding sequence was subcloned from the β-GFP plasmid , into the pBI121 binary vector (Clontech Laboratories, USA), by replacing the β - glucuronidase coding sequence into the BglII/SacI restriction sites as described by Zottini et al. . ..

    Article Title: Triticum aestivum WRAB18 functions in plastids and confers abiotic stress tolerance when overexpressed in Escherichia coli and Nicotiania benthamiana
    Article Snippet: .. The cDNA was then digested with XbaI and SacI, purified, and ligated to linearized pBI121 binary vector (Clontech) containing the powerful Cauliflower mosaic virus (CaMV) 35S promoter and the kanamycin resistance gene. .. 10 ng of the recombinant plasmid was added into 50 μL Agrobacterium tumefaciens (GV3101) competent cells in 0.5 mL eppendorf tube and mixed by tapping the tube.

    Article Title: Cell cycle stage-specific differential expression of topoisomerase I in tobacco BY-2 cells and its ectopic overexpression and knockdown unravels its crucial role in plant morphogenesis and development.
    Article Snippet: DNA topoisomerases catalyze the inter-conversion of different topological forms of DNA.. Cell cycle coupled differential accumulation of topoisomerase I (Topo I) revealed biphasic expression maximum at S-phase and M/G1-phase of cultured synchronized tobacco BY-2 cells.. This suggested its active role in resolving topological constrains during DNA replication (S-phase) and chromosome decondensation (M/G1 phase).

    Polymerase Chain Reaction:

    Article Title: Functional characterization of a type 2 metallothionein gene, SsMT2 , from alkaline-tolerant Suaeda salsa
    Article Snippet: .. The PCR fragments were digested with Bam HI and Sac I and then ligated into the Bam HI/ Sac I site of pBI121 binary vector (Takara, Tokyo, Japan), the plasmid DNAs of pBI121-SsMT2 was transformed into the Agrobacterium tumefaciens strain EHA105 (Takara, Tokyo, Japan) and then Arabidopsis (ecotype: Columbia) was transformed using the floral dip method . .. To examine the expression pattern of the SsMT2 gene in different organs of S . salsa plant, total RNA was isolated from roots, leaves, shoots, flowers and seeds respectively using RNeasy Plant Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions.

    Transformation Assay:

    Article Title: Functional characterization of a type 2 metallothionein gene, SsMT2 , from alkaline-tolerant Suaeda salsa
    Article Snippet: .. The PCR fragments were digested with Bam HI and Sac I and then ligated into the Bam HI/ Sac I site of pBI121 binary vector (Takara, Tokyo, Japan), the plasmid DNAs of pBI121-SsMT2 was transformed into the Agrobacterium tumefaciens strain EHA105 (Takara, Tokyo, Japan) and then Arabidopsis (ecotype: Columbia) was transformed using the floral dip method . .. To examine the expression pattern of the SsMT2 gene in different organs of S . salsa plant, total RNA was isolated from roots, leaves, shoots, flowers and seeds respectively using RNeasy Plant Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions.

    Expressing:

    Article Title: Mitochondria Change Dynamics and Morphology during Grapevine Leaf Senescence
    Article Snippet: .. For the expression of the GFP targeted to mitochondria, the β-GFP coding sequence was subcloned from the β-GFP plasmid , into the pBI121 binary vector (Clontech Laboratories, USA), by replacing the β - glucuronidase coding sequence into the BglII/SacI restriction sites as described by Zottini et al. . ..

    Sequencing:

    Article Title: Mitochondria Change Dynamics and Morphology during Grapevine Leaf Senescence
    Article Snippet: .. For the expression of the GFP targeted to mitochondria, the β-GFP coding sequence was subcloned from the β-GFP plasmid , into the pBI121 binary vector (Clontech Laboratories, USA), by replacing the β - glucuronidase coding sequence into the BglII/SacI restriction sites as described by Zottini et al. . ..

    Purification:

    Article Title: Triticum aestivum WRAB18 functions in plastids and confers abiotic stress tolerance when overexpressed in Escherichia coli and Nicotiania benthamiana
    Article Snippet: .. The cDNA was then digested with XbaI and SacI, purified, and ligated to linearized pBI121 binary vector (Clontech) containing the powerful Cauliflower mosaic virus (CaMV) 35S promoter and the kanamycin resistance gene. .. 10 ng of the recombinant plasmid was added into 50 μL Agrobacterium tumefaciens (GV3101) competent cells in 0.5 mL eppendorf tube and mixed by tapping the tube.

    Virus:

    Article Title: Triticum aestivum WRAB18 functions in plastids and confers abiotic stress tolerance when overexpressed in Escherichia coli and Nicotiania benthamiana
    Article Snippet: .. The cDNA was then digested with XbaI and SacI, purified, and ligated to linearized pBI121 binary vector (Clontech) containing the powerful Cauliflower mosaic virus (CaMV) 35S promoter and the kanamycin resistance gene. .. 10 ng of the recombinant plasmid was added into 50 μL Agrobacterium tumefaciens (GV3101) competent cells in 0.5 mL eppendorf tube and mixed by tapping the tube.



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